Table 1 Bacterial strains and plasmids used in this study

DH5α

F⁻ Φ80dlac∆(lacZ)M15 ∆(lacZYA-argF) U169 endA1 recA1 hsdR17 (r_K⁻, m_K⁺) deoR thi-1 phoA supE44 λ⁻gyrA96 relA1; strain used for cloning procedures

[34]

C. glutamicum

ATCC 13032 (WT)

Biotin-auxotrophic wild type

[35]

WT_EtOH-Evo

Derivative of ATCC 13032 with (among others) G→A mutation 68 bp upstream of the ald (cg3096) start codon resulting from ALE

This work

WT::P01-ald

Derivative of ATCC 13032 with G→A mutation is introduced 68 bp upstream of the ald (cg3096) start codon

This work

Plasmids

pK19mobsacB

Kan^R; plasmid for allelic exchange in C. glutamicum; (pK18 oriV_E.c., sacB, lacZα)

[36]

pK19mobsacB-P₀₁-ald

Kan^R; derivative of pK19mobsacB for mutating the promoter in front of ald (cg3096). The G→A mutation is introduced 68 pb upstream of the ALD start codon

This work