Sulfate limitation increases specific plasmid DNA yield and productivity in E. coli fed-batch processes

Gotsmy, Mathias; Strobl, Florian; Weiß, Florian; Gruber, Petra; Kraus, Barbara; Mairhofer, Juergen; Zanghellini, Jürgen

doi:10.1186/s12934-023-02248-2

Microbial Cell Factories

Table 2 Comparison of pDNA specific yields and volumetric yields in published studies

From: Sulfate limitation increases specific plasmid DNA yield and productivity in E. coli fed-batch processes

	Yield		Avg. vol.	Copy	Biomass	pDNA	Origin of	Selection	E. coli	Process
Reference	Specific	Volumetric	Productivity	Number	Conc.	Length	Replication	Pressure	Strain	Type
	[mg g\(^{-1}\)]	[mg L\(^{-1}\)]	[mg L\(^{-1}\) h\(^{-1}\)]	[plasmid cell\(^{-1}\)]	[gL\(^{-1}\)]	[kbp]
[54]	10.8^‡	58.3	3.9	NA	5.4	NA	NA	Ampicillin	BL21	Shake flask
[11]	15^†*	57^†	8.2^†‡	298^§	3.8^‡	5.4	pUC	Ampicillin	DH5\(\alpha\)	Shake flask
[13]	2.4^†	8.0	0.8^‡	86^§	3.3^‡	3.0	pUC	Kanamycin	BL21 recA-	Batch
[55]	2.4	19.9	0.3	42^§	8.3^‡	6.1	pUC	Ampicillin	VH34	Batch
[56]	2.4	30.0	2.7^†‡	42^§	12.5^‡	6.1	pUC	Ampicillin	VH33	Batch
[19]	7.0	230	17.7	192^§	34	3.9	pUC	Kanamycin	DH5\(\alpha\)	Batch
[20]	7.9	35.9	2.3^‡	529^§	4.6^†	1.6	pUC	Ampicillin	DH5\(\alpha\)	Batch
[57]	11.3	39.4	1.1^†‡	176^§	3.5^†	6.9	pUC	Ampicillin	DH5\(\alpha\)	Batch
[17]	17.1^‡	60	0.7^‡	286^§	3.5	6.4	ColE1	None	JM109	Batch
[16]	19.1	141	NA	553^§	7.4^‡	3.7	pUC	Kanamycin	GALG20	Batch
[18]	32.4	102.8	NA	526^§	3.2	6.6	pBR322	Ampicillin	VH33	Batch
[10]	6.9^‡	NA	NA	107^§	NA	6.9	pUC	Ampicillin	W3110	Continuous
[58]	1.6	33	3.0	43^§	20.6^‡	4.0	pUC	None	DH5\(\alpha\)	Exp. fed-perfusion
[59]	10.1	74.8	3.1^‡	451^§	8.3	2.4	pUC	Kanamycin	PFC	Fed-batch in shake flask
[12]	1.2	50	4.1	21^§	41	6.1	pUC	Ampicillin	VH33	Exp. fed-batch
[60]	7.6	25.6	NA	118^§	3.4^‡	6.9	pUC	Ampicillin	DH5\(\alpha\)	Exp. fed-batch
[57]	9.1	44	1.3^‡	141^§	4.8^‡	6.9	pUC	Ampicillin	DH5\(\alpha\)	Exp./lin. fed-batch
[61]	9.5 ^*	250	11.3^†‡	217^§	26.4^*	4.7	f1	Kanamycin	DH5\(\alpha\)	Lin. fed-batch
[62]	9.8	140	3.9^†‡	284^§	14.3^‡	3.7	ColE1	Kanamycin	GALG20	Exp. fed-batch
[63]	31.2^*	1923	72.4^‡	NA	62^‡*	NA	pUC	Ampicillin	BL21 recA\(^-\)	Lin. fed-batch
[64]	15.5	759	31.2	426^§	49^‡	3.9	pUC	Kanamycin	DH5\(\alpha\)	Exp. fed-batch
[8]	25.9	939	35.1	1142	36^‡	3.5	ColE1	None	JM108murselect	Exp. fed-batch
[62]	55.8^†‡*	2177	41^†‡	965^§	39^†*	6.2	ColE1	Kanamycin	GALG20	Exp. fed-batch
[24]	51	2200	NA	841^§	43^‡	6.5	pUC	Kanamycin	DH5\(\alpha\)	Exp. fed-batch
[65]	65^*	1830	42^†‡	1659^§	28^‡*	4.2	pUC	None	NTC4862	Exp. fed-batch
[25]	68^*	2590	NA	1176^§	38^‡*	6.2	pUC	Kanamycin	DH5\(\alpha\)	Exp. fed-batch
control	57.1\(\pm 0.7\)	2528\(\pm 68\)	81.5\(\pm 2.2\)	510^§	44.2\(\pm 0.9\)	12.0	pUC	None	JM108	Lin. fed-batch
\({\text {SO}_4^{2-}}\) limited	73.9\(\pm 4.6\)	2788\(\pm 161\)	89.9\(\pm 5.2\)	660^§	38.2\(\pm 1.0\)	12.0	pUC	None	JM108	Lin. fed-batch

The last two rows show the results of the experiments of this study (all pDNA conformations). Values marked with \(\dag\) are estimated from published figures. Values marked with \(\ddag\) are calculated from reported values. Values marked with \(*\) are converted from OD\(_\text {600}\) to cell dry mass (0.33g/L/OD\(_{600}\), BNID109838 [53]). Values marked with §are estimated with 50% GC content and an E. coli cell mass of 110 fg (BNID100498 [53]). Values reported as NA were not accessible

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ISSN: 1475-2859

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