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Table 4 Analysis of variance (ANOVA) for Response Surface Quadratic Model CCD) by mutant yeast Rhodotorula mucilaginosa E-54

From: Enhancement of novel Endo-polygalacturonase expression in Rhodotorula mucilaginosa PY18: insights from mutagenesis and molecular docking

Source

Sum of squares

Df

Mean square

F-value

p-value

 

Model

12,943.44

14

924.53

24.46

 < 0.0001

Significant

A-Ph

10,133.66

1

10,133.66

268.07

 < 0.0001

 

B-Inc. time

339.56

1

339.56

8.98

0.0090

 

C-xylose

124.40

1

124.40

3.29

0.0897

 

D-malt extract

867.78

1

867.78

22.96

0.0002

 

AB

444.37

1

444.37

11.75

0.0037

 

AC

9.61

1

9.61

0.2542

0.6214

 

AD

163.07

1

163.07

4.31

0.0554

 

BC

0.5852

1

0.5852

0.0155

0.9026

 

BD

57.38

1

57.38

1.52

0.2369

 

CD

29.76

1

29.76

0.7872

0.3890

 

A2

11.19

1

11.19

0.2961

0.5943

 

B2

381.63

1

381.63

10.10

0.0062

 

C2

55.70

1

55.70

1.47

0.2436

 

D2

113.32

1

113.32

3.00

0.1039

 

Residual

567.04

15

37.80

   

Lack of fit

531.78

10

29.21

1.34

0.3924

Not significant

Pure error

35.27

5

7.05

   

Cor Total

13,510.48

29

    
  1. Factor coding is coded. Sum of squares is Type III—Partial
  2. The Model F-value of 24.46, with a very low probability (0.01%) of this F-value being attributed to random error
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