Fig. 2

Time-course effect of acetic, butyric and octanoic Acids in yeast intracellular pH. Growth curve and intracellular pH (pHi, black and red crosses, and grey line) variation during S. cerevisiae BY4741pHl growth in MM (pH 4.5) in the absence (a) or presence of 53 mM (3.23 g/L) acetic acid (b), 11 mM (0.97 g/L) butyric acid (c) or 0.43 mM (0.06 g/L) octanoic acid (d) at 30 ℃ with orbital agitation. Cell growth was based on OD_600nm (dark blue circles). Results from two representative experiments performed to obtain the pHi profiles are shown as the values of each replicate (black and red crosses) and as the calculated average of the two values (grey line)