Fig. 3
Equivalent growth inhibitory concentrations of the weak acids used for the chemogenomic analysis. Spot growth of S. cerevisiae BY4741 cultivated in YPD solid medium (pH 4.5) either or not supplemented with acetic acid (75 mM C2), butyric acid (14 mM C4), or octanoic acid (0.3 mM C8). Exponentially-growing cell suspensions of BY4741 (OD600nm of 1 ± 0.05) were diluted to an OD600nm of 0.5 ± 0.005 (a) and this suspension was used to prepare 1:2 (b), 1:4 (c), 1:20 (d), 1:100 (e), 1:500 (f), 1:2500 (g) and 1:12,500 (h) diluted suspensions. Spot growth was registered after 48 h of incubation at 30 ℃. This picture is a representative example of several independent growth experiments