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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida

Fig. 1

Metabolic pathways of aliphatic diols in engineered P. putida KT2440. P. putida KT2440 wild type-based strains (A–C) and P. putida KT2440-AA-based strains (D–F) were cultivated in mineral salts medium (MSM) supplemented with 1,6-hexanediol, 1,7-heptanediol, or 1,8-octanediol in concentrations that are C-mol equivalent to 30 mM 1,6-hexanediol. Depending on the background strain, the mcl-diols are either metabolized via the HA-CoA-activating (I) or DCA-CoA-activating (II) pathway which required the expression of the heterologous dcaAKIJP cluster in P. putida KT2440-AA (genomic modifications in grey). Depending on the chain-length of the diol (dashed lines), namely C = 4 (1,4-butanediol), C = 5 (1,5-pentanediol), C = 6 (1,6-hexanediol), C = 7 (1,7-heptanediol), and C = 8 (1,8-octanediol) and the respective pathway, different central metabolites are obtained. The results of single mutant cultivation are shown in Fig. S2. Growth was monitored using a Growth Profiler. Error bars indicate the standard error of the mean (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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