Fig. 4

Growth of KT2440-AA strains on 1,7-heptanediol and on a mcl-DCA -diol mixture. All strains were cultivated in MSM containing 25.7 mM 1,7-heptanediol (A) or a mixture consisted of adipate, pimelate, suberate, azelate, sebacate, 1,6-hexanediol (HDO), 1,7-heptanediol, and 1,8-octanediol (ODO) with concentrations of 3 mM each (B). Red indicates the inability of the strain to metabolize the substrate. Yellow indicates metabolism via the HA-CoA-activating pathway, whereas blue indicates that the substrate was metabolized via the DCA-CoA-activating pathway. Potential activity of both pathways is indicated as color gradient. All mutations shown are in the KT2440-AA strain. Error bars indicate the standard error of the mean (n = 3)