Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida

Table 1 Production of mcl-PHA by engineered P. putida KT2440-AA secG^G70R, gcdR^G154Dfrom different substrates

Substrate	CDW (g L⁻¹)	PHA (%)	C₆ (%)	C₈ (%)	C₁₀ (%)	C₁₂ (%)
adipate	0.62 ± 0.05	15.7 ± 1.05	14.3 ± 3.2	24.1 ± 0.2	57.8 ± 3.2	3.8 ± 0.2
pimelate	0.62 ± 0.02	4.3 ± 0.12	14.2 ± 0.2	32.3 ± 0.3	47.9 ± 0.2	5.6 ± 0.0
suberate	0.47 ± 0.00	0.4 ± 0.01	n. d.	5.0 ± 1.1	48.4 ± 1.6	46.6 ± 3.4
azelate	0.47 ± 0.01	0.4 ± 0.02	n. d.	n. d.	48.8 ± 3.4	51.2 ± 3.4
sebacate	0.52 ± 0.03	0.6 ± 0.01	n. d.	9.9 ± 0.2	55.8 ± 0.7	34.3 ± 0.4
1,6-hexanediol	0.57 ± 0.03	10.0 ± 0.6	10.0 ± 0.5	33.4 ± 0.2	52.9 ± 0.1	3.8 ± 0.2
1,7-heptanediol	0.57 ± 0.01	3.4 ± 0.01	12.8 ± 0.7	30.0 ± 0.0	49.7 ± 0.2	7.5 ± 0.5
1,8-octanediol	0.43 ± 0.01	0.5 ± 0.00	4.7 ± 0.5	7.2 ± 0.1	49.1 ± 2.8	39.0 ± 2.0
mock hydrolysate	0.89 ± 0.02	0.3 ± 0.01	n. d.	n. d.	60.9 ± 1.1	39.1 ± 1.1

The CDW, PHA content, and relative monomer composition of mcl-PHA are shown. The strain was cultivated in MSM supplemented with C-mol equimolar concentrations to 30 mM of adipate using a C:N ratio of 30:1. The mock hydrolysate consisted of 5 mM of each individual substrate. Error values are calculated as standard deviations (n = 2). Exemplary GC chromatograms are shown in Fig. S7

ISSN: 1475-2859