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Table 2 Production of PHB by engineered P. putida KT2440-AA secGG70R, gcdRG154D carrying plasmid pS6311·PHB from different substrates

From: Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida

Substrate

CDW (g L−1)

PHB (%)

Adipate

1.52 ± 0.05

1.16 ± 0.06

Pimelate

0.23 ± 0.01

15.14 ± 0.05

Suberate

1.52 ± 0.04

1.56 ± 0.19

Azelate

1.47 ± 0.01

6.53 ± 0.92

Sebacate

1.52 ± 0.02

2.35 ± 0.07

1,6-Hexanediol

1.29 ± 0.14

3.59 ± 0.01

1,7-Heptanediol

0.26 ± 0.01

21.86 ± 2.77

1,8-Octanediol

0.56 ± 0.01

2.20 ± 0.76

Mock hydrolysate

1.16 ± 0.01

3.59 ± 0.01

  1. The CDW and PHB content are shown. The strain was cultivated in MSM supplemented with C-equimolar concentrations of pure substrates and 1 mM cyclohexanone as inducer for phaCAB expression. The strain was cultivated in MSM supplemented with C-mol equimolar concentrations to 30 mM of adipate using a C:N ratio of 30:1. The mock hydrolysate consisted of 5 mM of each individual substrate. Error values are calculated as standard deviations (n = 2). Exemplary GC chromatograms are shown in Fig. S7
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Microbial Cell Factories

ISSN: 1475-2859

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