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Table 1 Table summarizing CCB and PCB characteristics after batch cell bank production in terms of production conditions, identity, and purity: (i) the conditions of preparation for CCB and PCB, i.e. medium types and oxygenation, (ii) the parameters used to verify that bacterial identity is maintained while switching from the CCB and PCB, i.e. comparison of cell size, cell morphology, cytometry spectra, number of magnetosomes per cell, mean magnetosome size, magnetosome size distribution, multiplex PCR of MSR1 16S RNA, between CCB and PCB, (iii) the parameters highlighting bacterial purity in PCB and CCB, i.e. bacterial integrity established by cultivating MSR1 bacteria on LB agar plate and observation of a suspension of MSR1 bacteria to verify the absence of contaminants

From: Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes

  

CCB

PCB

Production

Medium

Medium 380 (DSMZ)

Minimal medium

Oxygen concentration

Anaerobic conditions

Microaerobic conditions

Identity

Cell size (µm)

1.20 ± 0.25

1.21 ± 0.25

Morphology

Spirilla shape with a single magnetosome chain at midcell

Bend shape with a single magnetosome chain at midcell

Cytometry analysis

Similar size and granulometry

Number of magnetosome per cell

21.19 ± 5.55

14.44 ± 6.28

Mean magnetosome size (nm)

39.99 ± 9.59

37.73 ± 6.71

Magnetosome size distribution (FWHM)

20.21

11.24

MSR1 16S RNA Multiplex PCR

2 fragments of 209 and 326 pb

Identity confirmed

2 fragments of 209 and 326 pb

Identity confirmed

Purity

Integrity on LB agar plate

Free of contaminant

Free of contaminant

Optic microscopic analysis

Free of contaminant

Free of contaminant