Skip to main content

Table 2 Summary of bacterial growth parameters and magnetosome properties obtained after 140 h of cultivation, corresponding to ~ 5 bacterial generation, in a 7.5 L bioreactor fed-batch culture of MSR1 bacteria derived from unfrozen CCB, unfrozen PCB and 16-month stored PCB to evaluate PCB stability after long-term storage and compare production performance with CCB, i.e. optical density of bacterial suspension measured at 565 nm (OD565), biomass (g), generation time (h), total intracellular iron in mg of intracellular iron per liter of growth medium, quantity of internalized iron inside cells in mg per gram of dried cell weight, volume of feed medium injected during culture, mass of dried magnetosomes purified, yield of magnetosome production in mg of dried magnetosomes purified per liter of growth medium, total intracellular iron in the form of magnetosome in percentage and number of magnetosomes per bacterium, magnetosome size (nm)

From: Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes

Starting culture inoculum

Unfrozen CCB

Unfrozen PCB

16 months stored PCB

OD565

3.90 ± 0.28

2.83 ± 0.22

2.91 ± 1.16

Biomass (g)

6.76 ± 0.25

5.28 ± 0.39

5.64 ± 1.59

Generation time (h)

18.26 ± 0.26

17.96 ± 1.55

15.71 ± 0.31

Total intracellular iron concentration (mg/L)

9.53 ± 0.73

8.10 ± 1.64

8.86 ± 1.44

Iron ratio per gram of DCW (mgFe/g)

6.87 ± 0.27

7.64 ± 0.98

8.39 ± 3.66

Volume of feed injected (mL)

235 ± 6

212 ± 26

235 ± 5

Magnetosome mass (mg)

7.05 ± 0.64

24.90 ± 5.66

28.40 ± 14.14

Magnetosome volumetric yield (mg/L)

1.45 ± 0.13

4.98 ± 1.13

5.66 ± 2.82

Total intracellular iron as magnetosome (%)

9.26 ± 0.49

44.38 ± 1.14

38.65 ± 14.88

Number of magnetosome per cell

12.11 ± 4.79

24.15 ± 13.18

21.01 ± 9.43

Magnetosome size (nm)

35.16 ± 6.94

36.94 ± 6.07

35.50 ± 7.11