Fig. 1
Cloning and heterologous production of rLO. A Gel 1% agarose of PCR amplification of LO gene. Line 1: DNA marker; Line 2: Band of LO gene amplified from the cDNA of T. harzianum. B Gel 1% agarose of the vector cloned pGEX-4T1-LO. C Gel 1% agarose of the fragments of plasmid pGEX-4T1 and LO gene after plasmid cleavage with the restriction enzymes BamHI and EcoRI confirming the fragments with the expected sizes. D SDS-PAGE of rLO purification. Line 1: Protein marker; Line 2: Crude extract; Line 2 to 3: Wash samples; Line 4 to 15: Elution samples. The band between 66.2 and 116 kDa is rLO. The band below is GST. E Gel SDS-PAGE of rLO (Line 2) and rLOGST− (Line 3) after treatment of rLO with Thrombin Protease for GST removal. Line 1: Protein Marker. Molecular mass standards: β-galactosidase, 116 kDa; bovine serum albumin, 66.2 kDa; ovalbumin, 45 kDa; lactate dehydrogenase, 35 kDa; REase Bsp98I, 25 kDa; β-lactoglobulin, 18.4 kDa; lysozyme, 14.4 kDa