Fig. 2

Evaluation of cytotoxicity of rLO in Jurkat and PBMC cells. Jurkat cells (A, B) and PBMC (C, D) were treated with doses of 1 to 5 mU/mL of rLO, and after 24 h of incubation, the cells were stained with annexin V and/or PI following the flow cytometry analysis. Arsenic trioxide (ATO) was considered a positive control, and the negative control was growth medium only. A and C The concentration of viable cells was determined by the number of negative cells for unstained cells as measured by flow cytometry. B and D Percentage of cells stained for early apoptosis (Annexin V +) and late apoptosis (Annexin V/PI + / +) after the treatment with different doses of rLO