Fig. 3

Dose- and time-dependent response of rLO treatment on apoptotic and necrotic events in Jurkat cells. A Time-lapse microscopy of rLO-treated Jurkat cells. Photographs were taken every 3 min for 22 h. In the figure, the photos correspond to 0 and 18 h after stimulation with rLO 1 mU/mL. B Evaluation of growth inhibition of Jurkat cell by Population Doubling Level (PDL). Jurkat cells were treated with different concentrations of rLO, and growth inhibition was evaluated by counting cells in a Neubauer chamber every 48 h. Negative control: untreated cells. Positive control: cells treated with ATO. C Comparative analysis by Heatmap of the viability of Jurkat cells treated with rLO over time. Jurkat cells were treated with doses of 0.25, 0.5, 1, 2, and 4 mU/mL of rLO. After 6, 10, and 18 h, propidium iodide was added to the samples, and viability was determined by analysis of PI incorporation by flow cytometry. CTRL (-): Medium/untreated cells. Positive control: ATO