Fig. 4

Biochemical characterization of the rTFPs a. Stability of rec-αCTX and rec-Hannalgesin upon prolonged storage at 4 °C, analyzed with non-reducing SDS-PAGE; b. ‘Concentrate to dry’ strategy (right, with black arrow pointing to the monomeric species) efficiently removed multimeric species that were hard to be separated with the mono S column (left, in which the refolded product was not ‘concentrate to dry’, grey arrow); c. Gel filtration analysis of rec-αBtx, rec-mPate B and rec-κBtx. d. Native gel shift assay of various rTFPs with HAP peptide. e. Native gel shift assay of rec-α1ECD with native αCTX, rec-αCTX and rec-Hannalgesin