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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Recombinant L. lactis vaccine LL-plSAM-WAE targeting four virulence factors provides mucosal immunity against H. pylori infection

Fig. 2

Expression and identification of LL-plSAM-WAE. (a) SDS‒PAGE. M, protein marker; Lanes 1 and 3 included the supernatant of LL-plSAM-WAE after induction with nisin, whereas lanes 2 and 4 contained the lysate supernatants of LL-plSAM-WAE after induction with nisin. (b) Western blot. The presence of the SAM-WAE protein may be identified by the use of a mouse WAE antibody. (c) Immunofluorescence examination. (d) Various doses of LL-plSAM-WAE or LL-plSAM were coated on ELISA plates. (e) SAM-WAE (5 µg/mL), LL-plSAM (5 × 108 CFUs/well), and LL-plSAM-WAE (5 × 108 CFUs/well) were coated on ELISA plates

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Microbial Cell Factories

ISSN: 1475-2859

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