Fig. 3

Detection of T-cell immune responses in the spleen. One week following the last immunization, spleens were taken. (a) The marginal zones of spleen sections from the PBS, SAM-WAE, and LL-plSAM-WAE groups were immunofluorescently stained in a representative manner. (b) The Relative fluoresence intensity of CD4. (c) The single cell suspensions from the spleen that were activated in vitro with 10 µg/mL CWAE are shown in representative ELISPOT data. (d) The quantity of splenic IFN-γ-producing cells that are antigen specific. (e) Representative FACS plots in the spleen are shown. (f) A total of 10⁴ CD4⁺ cells were used as a baseline to standardize the proportion and quantity of IFN-γ⁺, IL-4⁺, and IL-17⁺ cells in the spleen (g)