A genetic toolbox to empower Paracoccus pantotrophus DSM 2944 as a metabolically versatile SynBio chassis

Table 4 Oligonucleotides used in this study

Primer	Sequence (5’ → 3’)	Function	Refs.
UP01	ccgcgctggaggatcatccaTGAGAAGCCTCGCCTTCC	Amplification TS1 fragment, p∆PHB	This work
UP02	gccgggtttcGAGAACTGCGTCTTGCGC	Amplification TS1 fragment, p∆PHB	This work
UP03	cgcagttctcGAAACCCGGCGCGGCCAA	Amplification TS2 fragment, p∆PHB	This work
UP04	tcgttttccgggacgccggcGCCGGCGGGATAGTCGCG	Amplification TS2 fragment, p∆PHB	This work
UP05	GCCGGCGTCCCGGAAAAC	Amplification pEMG vector	This work
UP06	TGGATGATCCTCCAGCGCG	Amplification pEMG vector	This work
UP07	GTGCTGCAAGGCGATTAAGT	Control p∆PHB construct	This work
UP08	GCTAAAGCTGGAACGGGGAA	Control p∆PHB construct	This work
UP09	GCCCTTCCATGAATTGCGTC	Control p∆PHB construct	This work
UP10	GACCACCAAGCGAAACATCG	Control p∆PHB construct	This work
UP11	CGGTAGCCGCTTACTTGGC	Control of triparental mating knockout	This work
UP12	GCTATGACCATGATTACGCCGG	Control of triparental mating knockout	This work
UP13	GACGTTGTAAAACGACGGCC	Control of triparental mating knockout	This work
UP13	ACCTCGGCCGCAGTGAT	Control of triparental mating knockout	This work
UP14	TCTTCTTGCTAGGCGGGTTG	Verify P. pantotrophus DSM 2944 ∆PHB knockout	This work
UP15	GACGATTACGTCAGCGCCTA	Verify P. pantotrophus DSM 2944 ∆PHB knockout	This work
8F	AGAGTTTGATCCTGGCTCAG	16S Fwd	[83]
1492R	CGGTTACCTTGTTACGACTT	16S Rev	[84]

ISSN: 1475-2859