Table 1 List of strains, and plasmids used in this study
From: N-terminal truncation of PhaCBP-M-CPF4 and its effect on PHA production
Strain or plasmid | Description | Reference or source |
|---|---|---|
Strains | ||
 C. necator PHB−4 | C. necator with nonsense mutation of phaC1Cn at the 107th amino acid | DSM 541 |
 E. coli DH5α | General cloning strain. F− l− deoR supE44 hsdR17(rK− mK+) phoA recA1 endA1 gyrA96 thi-1 relA1 D(lacZYA-argF) U169 f80dlacZDM15 | Toyobo |
 E. coli S17-1 | E. coli strain for transconjugation of the plasmid into C. necator. recA, tra genes of plasmid RP4 integrated into the chromosome, auxotrophic for proline and thiamin | [38] |
Plasmids | ||
 pBBR1MCS2 | Broad-host-range cloning vector Kmr, mob, lacZα | [39] |
 pBBR1-ProCn | pBBR1MCS2 plasmid containing phaC1 promoter from C. necator | [8] |
 pBBR1-CBP-M-CPF4 | pBBR1MCS2 plasmid harbouring the phaC1 promoter of C. necator controlling the expression of phaCBP-M-CPF4 | [8] |
 pBBR1-CBP-M-CPF4 G8 | pBBR1MCS2 plasmid harbouring the phaC1 promoter of C. necator controlling the expression of phaCBP-M-CPF4 8–551 amino acids | This study |
 pBBR1-CBP-M-CPF4 A27 | pBBR1MCS2 plasmid harbouring the phaC1 promoter of C. necator controlling the expression of phaCBP-M-CPF4 27–551 amino acids | This study |
 pBBR1-CBP-M-CPF4 T74 | pBBR1MCS2 plasmid harbouring the phaC1 promoter of C. necator controlling the expression of phaCBP-M-CPF4 74–551 amino acids | This study |
 pBBR1-CBP-M-CPF4 D104 | pBBR1MCS2 plasmid harbouring the phaC1 promoter of C. necator controlling the expression of phaCBP-M-CPF4 104–551 amino acids | This study |