Table 2 List of primers used in this study
From: N-terminal truncation of PhaCBP-M-CPF4 and its effect on PHA production
Primer | Sequence | Source or reference |
|---|---|---|
HindIII_RBS_PhaC_BP-M-CPF4_G8_Fwd | AGT AAGCTT CAAAGGAGGGAAAGT ATGGGGAAAACAGGTGATTTGTGGTCAT | This study |
HindIII_RBS_PhaC_BP-M-CPF4_A27_Fwd | AGT AAGCTT CAAAGGAGGGAAAGT ATGGCCGCGGCACAGATTCAGCA | This study |
HindIII_RBS_PhaC_BP-M-CPF4_T74_Fwd | AGT AAGCTT CAAAGGAGGGAAAGT ATGACGGAAAACATGGCCGCCGA | This study |
HindIII_RBS_BP-M-CPF4_D104_Fwd | AGT AAGCTT CAAAGGAGGGAAAGT ATGGATCCCGATCTGCACGAAC | This study |
ApaI_PhaC_BP-M-CPF4_Rev | ATT GGGCCC CTACTTCTCCAAAACGTACGT | This study |
qPCR_ PhaC_BP-M-CPF4_Forw | CACCACCCACCAGGATTTCAGC | This study |
qPCR_ PhaC_BP-M-CPF4_Rev | GTAATTCCATAGCAGGTCATTGGCCCG | This study |
qPCR_16S_Forw | GTGGCGAACGGGTGAGTAATACATCG | This study |
qPCR_16S_Rev | CCAGCTACTGATCGTCGCCTTGG | This study |