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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications

Fig. 3

Expression of hegf in P. pastoris GS115, H001 and H002 under PAOX and PGAP respectively. (a) SDS-PAGE analysis of purified His-tagged hegf. lane M: protein marker; lane 1: GS115/pGAPZα A-hegf; lane 2: H001/pGAPZα A-hegf; lane 3: H002/pGAPZα A-hegf; lane 4: GS115/pPICZα A-hegf; lane 5: H001/pPICZα A-hegf; lane 6: H002/pPICZα A-hegf. (b) The highest hegf contents quantified by high-performance chromatographic liquid chromatography. (c) Changes in hegf content secreted by H002-pPICZ α A-hegf during cultivation. Mean ± SEM are shown (n = 6). Error bars indicate standard deviation. *** represented p < 0.001 and **** represented p < 0.0001

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Microbial Cell Factories

ISSN: 1475-2859

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