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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications

Fig. 6

Expression of ergothioneine (ERG) in P. pastoris GS115, H001 and H002 under PAOX and PGAP respectively. (a) Two different pathways for ERG biosynthesis in bacteria and fungi respectively. EgtABCDE are responsible for ERG synthesis in bacteria, and Egt12 are responsible for ERG synthesis in fungi. Histidine (His); Hercynine (HER); Glutamate (Glu); Cysteine (Cys); γ-glutamylcysteine (γGC); S-hercynyl-c-glutamylcysteine sulfoxide (γGC-HER); S-hercynylcysteine sulfoxide (Cys-HER); Ergothioneine (ERG). (b) The highest ERG levels of recombinant strains employing different chassis hosts and gene combinations. (c) Changes in ERG of H001-pGAPZ A-PGAP-egt1E during cultivation. Mean ± SEM are shown (n = 6). Error bars indicate standard deviation. * Represented p < 0.05, ** represented p < 0.01, *** represented p < 0.001 and **** represented p < 0.0001

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Microbial Cell Factories

ISSN: 1475-2859

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