Table 1 The host and recombinant strains used in the present study

Host strains

Escherichia coli JM109

wild-type E. coli

Pichia pastoris GS115

wild-type Pichia pastoris

H001

GS115 mutant in PAS_chr1-3_0225 gene

H002

GS115 mutant in PAS_chr2-1_0661 gene

GFP-expressing strains

GS115-pGAPZ A-gfp

GS115 harboring gfp under P_GAP

GS115-pPICZ A-gfp

GS115 harboring gfp under P_AOX

H001-pGAPZ A-gfp

H001 harboring gfp under P_GAP

H002-pGAPZ A-gfp

H002 harboring gfp under P_GAP

H001-pPICZ A-gfp

H001 harboring gfp under P_AOX

H002-pPICZ A-gfp

H002 harboring gfp under P_AOX

hegf-expressing strains

GS115-pGAPZα A-hegf

GS115 harboring hegf under P_GAP and α factor

GS115-pPICZα A-hegf

GS115 harboring hegf under P_AOX and α factor

H001-pGAPZα A-hegf

H001 harboring hegf under P_GAP and α factor

H002-pGAPZα A-hegf

H002 harboring hegf under P_GAP and α factor

H001-pPICZα A-hegf

H001 harboring hegf under P_AOX and α factor

H002-pPICZα A-hegf

H002 harboring hegf under P_AOX and α factor

SAM-biosynthesis strains

GS115-pGAPZ A-sam2

GS115 harboring sam2 under P_GAP

GS115-pPICZ A-sam2

GS115 harboring sam2 under P_AOX

H001-pGAPZ A-sam2

H001 harboring sam2 under P_GAP

H002-pGAPZ A-sam2

H002 harboring sam2 under P_GAP

H001-pPICZ A-sam2

H001 harboring sam2 under P_AOX

H002-pPICZ A-sam2

H002 harboring sam2 under P_AOX

ERG-biosynthesis strains

GS115-pGAPZ A-egt12

GS115 harboring egt12 under P_GAP

GS115-pGAPZ A-egt1E

GS115 harboring egt1E under P_GAP

GS115-pPICZ A-egt12

GS115 harboring egt12 under P_AOX

GS115-pPICZ A-egt1E

GS115 harboring egt1E under P_AOX

H001-pGAPZ A-egt12

H001 harboring egt12 under P_GAP

H001-pGAPZ A-egt1E

H001 harboring egt1E under P_GAP

H001-pPICZ A-egt12

H001 harboring egt12 under P_AOX

H001-pPICZ A-egt1E

H001 harboring egt1E under P_AOX

H002-pGAPZ A-egt12

H002 harboring egt12 under P_GAP

H002-pGAPZ A-egt1E

H002 harboring egt1E under P_GAP

H002-pPICZ A-egt12

H002 harboring egt12 under P_AOX

H002-pPICZ A-egt1E

H002 harboring egt1E under P_AOX