Table 1 The responses for the Plackett-Burman design for Curvularia soli AS21 ON076460 of the un-irradiated control and gamma irradiated at 1.0 kGy to explore the significant effect of different factors on the melanin productivity
Run | Metals | Carbon source | Nitrogen source | Temp | pH | Incubation/day | Illum | Shaking | Medium type | Tyrosine g/L | NaCl g/L | Absorbance at λ = 480 nm | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | 1 KGy | ||||||||||||
1 | Zn | Starch | YE | 25 | 6 | 21 | − | − | DOX | 0.5 | 3 | 2.865 | 2.920 |
2 | Zn | Fructose | YE | 25 | 8 | 15 |  +  | − | PDA | 1 | 3 | 1.929 | 2.938 |
3 | Cu | Starch | Peptone | 30 | 6 | 15 | − | − | PDA | 1 | 3 | 2.688 | 3.285 |
4 | Zn | Fructose | Peptone | 30 | 8 | 15 | − |  +  | DOX | 0.5 | 3 | 2.855 | 3.065 |
5 | Cu | Fructose | YE | 30 | 6 | 21 |  +  |  +  | DOX | 1 | 3 | 2.732 | 1.992 |
6 | Zn | Starch | YE | 30 | 8 | 21 | − |  +  | PDA | 1 | 1.5 | 2.428 | 3.327 |
7 | Cu | Fructose | Peptone | 25 | 8 | 21 | − | − | DOX | 1 | 1.5 | 1.860 | 2.127 |
8 | Zn | Starch | Peptone | 25 | 6 | 15 |  +  |  +  | DOX | 1 | 1.5 | 3.054 | 3.325 |
9 | Zn | Fructose | Peptone | 30 | 6 | 21 |  +  | − | PDA | 0.5 | 1.5 | 3.042 | 3.376 |
10 | Cu | Starch | Peptone | 25 | 8 | 21 |  +  |  +  | PDA | 0.5 | 3 | 1.699 | 2.080 |
11 | Cu | Starch | YE | 30 | 8 | 15 |  +  | − | DOX | 0.5 | 1.5 | 2.087 | 2.128 |
12 | Cu | Fructose | YE | 25 | 6 | 15 | − |  +  | PDA | 0.5 | 1.5 | 2.750 | 3.285 |