Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

Keita, Valériane Malika; Lee, Yi Qing; Lakshmanan, Meiyappan; Ow, Dave Siak-Wei; Staniland, Paul; Staniland, Jessica; Savill, Ian; Tee, Kang Lan; Wong, Tuck Seng; Lee, Dong-Yup

doi:10.1186/s12934-024-02336-x

Microbial Cell Factories

Table 3 Effects of glycerol concentration and pH of sweetwater on yeast growth

From: Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

Strains	Concentration effect				pH effect
	SW_35>1.5SC		SW₃₅SC		SW₁₅ pH 6.09		SW₁₅ pH 3.95
	t_mid (h)	CC	t_mid (h)	CC	t_mid (h)	CC	t_mid (h)	CC
B. californica NRRL Y-1680	9.45 ± 0*	1.4 ± 0*	19.75 ± 0.08	1.62 ± 0.01	21.23 ± 0.57	1.05 ± 0.04	22.68 ± 2.85	1.05 ± 0.02
C. xylofermentans BCC 30719	23.7 ± 1.82	0.96 ± 0.08	60.82 ± 4.33	1.91 ± 0.04	39.67 ± 2.92	1.2 ± 0.03	49.2 ± 6.36	0.99 ± 0.11
C. curvatus NRRL Y-1511	21.26 ± 1.15	1.46 ± 0.01	64.98 ± 2.76	1.42 ± 0.01	41.53 ± 0.88	1.24 ± 0.04	93.65 ± 8.76	1.29 ± 0.24
C. saturnus NRRL YB-4312	13.43 ± 2.48	1.45 ± 0.03	26.13 ± 0.73	1.68 ± 0.04	29.25 ± 0.45	1.32 ± 0.02	31.29 ± 0.48	1.29 ± 0.02
L. lipofer DSM 70305	59.51 ± 3.32	0.89 ± 0.09	171.86 ± 27.74	0.69 ± 0.25	79.73 ± 4.38	0.8 ± 0.05	66.07 ± 3.93	0.35 ± 0.06
L. starkeyi NRRL Y-1388	47.11 ± 0.56	0.99 ± 0.02	-	-	66.81 ± 1.62	0.75 ± 0.04	67.86 ± 1.22	0.59 ± 0.06
L. elongisporus NRRL YB-4239	18.34 ± 0.08	1.33 ± 0.03	35.9 ± 3.4	1.85 ± 0.02	21.51 ± 0.95	1.08 ± 0.1	19.88 ± 0.18	1.15 ± 0.02
M. pulcherrima NRRL Y-5941-53	19.11 ± 0.18	1.38 ± 0.01	38.26 ± 0.28	1.67 ± 0.01	19.83 ± 0.09	1.41 ± 0.01	20.24 ± 0.17	1.42 ± 0.02
P. kudriavzevii NRRL Y-7551	22.13 ± 0.42	1.3 ± 0.02	36.96 ± 2.06	1.54 ± 0.01	27.56 ± 5.35	0.87 ± 0.26	54.06 ± 16.75	0.73 ± 0.11
R. glutinis NRRL Y-2502	24.18 ± 0.27	1.26 ± 0.02	103.3 ± 4.95	1.54 ± 0.03	27.18 ± 0.72	1.29 ± 0.03	54.23 ± 1.99	1.32 ± 0.02
R. mucilaginosa NRRL Y-17283	20.03 ± 0.07	1.3 ± 0.01	22.38 ± 0.1	1.67 ± 0.02	20.6 ± 0.37	1.1 ± 0.03	23.85 ± 0.97	1.02 ± 0.02
R. toruloides NRRL Y-6987	23.03 ± 1.76	1.57 ± 0.05	35.37 ± 0.23	1.73 ± 0.03	21.74 ± 0.35	1.34 ± 0.03	28.07 ± 1.83*	1.28 ± 0.02*
R. toruloides NRRL Y-1091	21.48 ± 0.62	1.35 ± 0.02	55.84 ± 0.66	1.78 ± 0.03	24.06 ± 1.75	1.15 ± 0.05	42.43 ± 2.47	1.13 ± 0.04
S. stipitis NRRL Y-7124	20.1 ± 0.61	1.45 ± 0	139.16 ± 0.64	1.82 ± 0.05	28.12 ± 6.3	1.3 ± 0.05	21.68 ± 1.7	1.08 ± 0.07
S. stipitis NRRL Y-11545	19.44 ± 1.13	1.35 ± 0.05	37.01 ± 0.43	1.76 ± 0.02	21.27 ± 0.32	1.23 ± 0.03	23.87 ± 2.81	1.09 ± 0.04
S. stipitis BCC 15191	23.76 ± 0.62	1.28 ± 0.02	33.69 ± 1.95	1.73 ± 0	20.71 ± 0.18	1.26 ± 0.03	21.18 ± 0.63	1.17 ± 0.1
S. occidentalis NRRL Y-2477	19.38 ± 0.24	1.12 ± 0.05	24.21 ± 0.09	1.73 ± 0.03	33.83 ± 1.08	1.45 ± 0.01	49.01 ± 1.93	1.24 ± 0.03
T. delbrueckii NRRL Y-866	18 ± 0.3	0.81 ± 0.06	26.64 ± 0.34	1.65 ± 0.04	21.56 ± 1.06	1.02 ± 0.06	21.09 ± 0.95	0.57 ± 0.07
W. anomalus NRRL Y-366	13.71 ± 1.88	1.37 ± 0.04	122.47 ± 3.04	1.98 ± 0.03	20.27 ± 0.07	1.41 ± 0.01	21.74 ± 0.35	1.84 ± 0.02
Y. lipolytica BCC 64401	20.43 ± 1.42*	1.39 ± 0*	19.99 ± 0.12	1.83 ± 0.03	21.59 ± 1.03	1.21 ± 0.04	20.75 ± 0.12	1.32 ± 0.01
Y. lipolytica Y203A	17.27 ± 1.52*	1.45 ± 0.05*	18.31 ± 0.32	1.69 ± 0.01	32.84 ± 10.87	1.33 ± 0.01	19.78 ± 0.3	1.32 ± 0.02

All the associated growth curves are available in Additional file 1: Figs. S6 and S7. An asterisk (*) indicates averages computed with less than 4 replicates

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