Table 1 Strains and plasmids used in this study

GRS

Prophage-cured ATCC13032 via in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752) and CGP3 (cg1890-cg2071) with additional in-frame deletions of the insertion elements ISCg1 and ISCg2

[29]

PC2

GRS with in-frame deletions of cg0414-0440, cg0635-0646, cg0704-0748, cg0822-0845, cg1018-1033, cg1172-1213, cg1219-1305 and cg1340-1352

[30]

ΔhdpAΔldh

C. glutamicum WT (ATCC13032) with in-frame deletions of hdpA (cg2474) and ldh (cg3219)

[22]

GRSΔhdpAΔldh

GRS with in-frame deletions of hdpA (cg2474) and ldh (cg3219)

This study

PC2ΔhdpAΔldh

PC2 with in-frame deletions of hdpA (cg2474) and ldh (cg3219)

This study

ΔhdpAΔldhΔdld

C. glutamicum WTΔhdpAΔldh with in-frame deletions of dld (cg1027)

This study

ΔhdpAΔldhΔmshA

C. glutamicum WTΔhdpAΔldh with in-frame deletions of mshA (cg0481)

This study

ΔhdpAΔldhΔoxyR

C. glutamicum WTΔhdpAΔldh with in-frame deletions of oxyR (cg2109)

This study

ΔhdpAΔldhΔcg0071

C. glutamicum WTΔhdpAΔldh with in-frame deletions of cg0071 (proposed gloB homolog)

This study

ΔhdpAΔldhΔcg1073

C. glutamicum WTΔhdpAΔldh with in-frame deletions of cg1073 (proposed gloA homolog)

This study

ΔhdpAΔldhΔcg1426

C. glutamicum WTΔhdpAΔldh with in-frame deletions of cg1426 (coding for Xi-class mycothiol S-transferase)

This study

ΔhdpAΔldhΔcg1482

C. glutamicum WTΔhdpAΔldh with in-frame deletions of cg1482 (proposed gloB homolog)

This study

ΔhdpAΔldhΔcg1856

C. glutamicum WTΔhdpAΔldh with in-frame deletions of cg1856 (proposed gloB homolog)

This study

Plasmids

pK19mobsacB

Mobilizable E. coli vector for the construction of deletion and

insertion mutants, Kan^R, sacB, lacZα with multiple cloning site,

oriV, oriT

[50]

pK19mobsacB-ΔhdpA

Kan^R, pK19mobsacB with the deletion construct for hdpA (cg2474)

[22]

pK19mobsacB-Δldh

Kan^R, pK19mobsacB with the deletion construct for ldh (cg3219)

[51]

pK19mobsacB-Δdld

Kan^R, pK19mobsacB with the deletion construct for dld (cg1027)

This study

pK19mobsacB-ΔmshA

Kan^R, pK19mobsacB with the deletion construct for mshA (cg0481)

This study

pK19mobsacB-ΔoxyR

Kan^R, pK19mobsacB with the deletion construct for oxyR (cg2109)

This study

pK19mobsacB-Δcg0071

Kan^R, pK19mobsacB with the deletion construct for cg0071 (proposed gloB homolog)

This study

pK19mobsacB-Δcg1073

Kan^R, pK19mobsacB with the deletion construct for cg1073 (proposed gloA homolog)

This study

pK19mobsacB-Δcg1426

Kan^R, pK19mobsacB with the deletion construct for cg1426 (coding for Xi-class mycothiol S-transferase)

This study

pK19mobsacB-Δcg1482

Kan^R, pK19mobsacB with the deletion construct for cg1482 (proposed gloB homolog)

This study

pK19mobsacB-Δcg1856

Kan^R, pK19mobsacB with the deletion construct for cg1856 (proposed gloB homolog)

This study

pEKEx3-mgsA-yqhD-gldA

Spec^R; derived from pEKEx3 for IPTG-inducible overexpression of mgsA, yqhD and gldA from E. coli with artificial ribosome binding site in front of each gene

[22]

pVWEx1

Kan^R; C. glutamicum/E. coli shuttle vector for regulated gene expression (P_tac, lacI^q, pHM1519, oriV_C.g., oriV_E.c.)

[52]

pVWEx1-oxyR

Kan^R; derived from pVWEx1 for IPTG-inducible overexpression of oxyR (cg2109) from C. glutamicum with artificial ribosome binding site in front of the gene

This study