Table 1 Composition of the reaction mixture [%] after 1, 3, 7 and 10 days obtained during biotransformation of flavokawain B (FB1) by HPLC
Strain | Compound | Retention time by HPLC [min] | Time of biotransformation [days] | |||
|---|---|---|---|---|---|---|
1 | 3 | 7 | 10 | |||
Metarhizium robertsii MU4 | FB1 | 9.578 | 33.6 | 10.4 | 4.6 | 4.3 |
FB2 | 9.075 | 0.8 | 0.6 | 0.6 | 1.2 | |
FB3 | 5.795 | 44.4 | 68.2 | 75.0 | 75.8 | |
FB4 | 4.641 | 2.0 | 2.3 | 2.5 | 2.5 | |
FB5 | 4.566 | 7.7 | 15.8 | 15.8 | 16.1 | |
u.c | - | 11.5 | 2.7 | 1.5 | 0.1 | |
Isaria tenuipes MU35 | FB1 | 9.578 | 89.0 | 75.5 | 63.4 | 53.5 |
FB2 | 9.075 | 4.1 | 6.2 | 12.1 | 9.8 | |
FB3 | 5.795 | 6.9 | 18.3 | 24.5 | 36.7 | |
Isaria fumosorosea KCh J2 | FB1 | 9.578 | > 99.9 | > 99.9 | > 99.9 | > 99.9 |
Isaria farinosa KCh KW1.1 | FB1 | 9.578 | > 99.9 | > 99.9 | > 99.9 | > 99.9 |
Beauveria bassiana KCh BBT | FB1 | 9.578 | 76.7 | 2.3 | 0.9 | 0.5 |
FB3 | 5.795 | 2.0 | 3.3 | 4.7 | 3.8 | |
FB4 | 4.641 | 2.2 | 16.0 | 16.0 | 20.0 | |
FB6 | 8.905 | 1.8 | 1.7 | 0.6 | 0.5 | |
FB7 | 5.729 | 10.5 | 43.0 | 43.5 | 32.5 | |
FB8 | 5.629 | 3.6 | 20.3 | 22.4 | 27.5 | |
u.c | - | 3.2 | 13.4 | 11.9 | 15.2 | |
Beauveria bassiana KCh J1.5 | FB1 | 9.578 | 13.6 | 4.6 | 1.5 | 1.6 |
FB3 | 5.795 | 2.4 | 0.0 | 0.0 | 0.0 | |
FB4 | 4.641 | 13.6 | 16.7 | 16.3 | 19.9 | |
FB6 | 8.905 | 0.8 | 0.1 | 0.05 | 0.0 | |
FB7 | 5.729 | 31.2 | 40.4 | 44.1 | 41.6 | |
FB8 | 5.629 | 27.7 | 30.2 | 30.3 | 31.2 | |
u.c | – | 10.7 | 8.0 | 7.8 | 7.7 | |
Beauveria bassiana KCh J1 | FB1 | 9.578 | 99.0 | 95.0 | 90.4 | 87.1 |
FB2 | 9.075 | 0.1 | 0.3 | 0.8 | 1.1 | |
FB6 | 8.905 | 0.9 | 4.7 | 9.8 | 11.8 | |
Beauveria caledonica KCh J3.3 | FB1 | 9.578 | 99.2 | 95.4 | 82.3 | 54.5 |
FB3 | 5.795 | 0.7 | 3.5 | 10.6 | 21.2 | |
FB4 | 4.641 | 0.0 | 0.4 | 2.7 | 9.8 | |
FB7 | 5.729 | 0.1 | 0.7 | 4.4 | 14.6 | |