Fig. 2

Trans-cinnamic acid consumption by P. putida CM12-5 expressing different trans-cinnamic acid decarboxylases. P. putida CM12-5 without plasmid or bearing the plasmid pPSC1 or pPSD1 were grown on M9 minimal medium with glucose supplemented with 0.25, 0.5 or 1 mM trans-cinnamic acid. tCA concentrations were determined at the beginning of the assay (blue bars) and after 24-h cultivation (yellow bars). ANOVA analysis was performed for three-group comparisons and Tukey test was carried out between paired groups to determine the statistically significance (p-value < 0.05). The results shown are the averages and standard deviations of three independent assays