Fig. 3

Trans-cinnamic acid and L-phenylananine consumption by P. putida CM12-5 (pPSC1) (A and B) and P. putida CM12-5 (pPALN) (C and D), respectively, at different pH and temperatures. For pH assays (A and C), cultures were grown at pH 5.8, pink open circles; pH 6.6, orange solid circles; pH 7.0, green open triangles; and pH 7.6, purple solid triangles. For temperature assays (B and D), cultures were grown in M9 minimal medium at pH 7.0 at 18 °C, pink open circles; 25 °C, orange solid circles; 30 °C, green open triangles; and 37 °C, purple solid triangles. Initial consumption rates correspond to the slope of a trendline during the first two hours of cultivation. The results shown are the averages and standard deviations of three independent assays