Fig. 4From: Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in PseudomonasBiosynthesis of styrene by P. putida derivatives from glucose. The strains used were P. putida CM12-5 (pPALN, pPSC1), P. putida CM12-5 (pPALN_C1) and P. putida CM12-5 as control. Cells were grown on M9 minimal medium with 0,5% (w/v) glucose as the sole C-source. Production of L-phenylalanine (blue bars), trans-cinnamic acid (yellow bars) and styrene (pink bars) was determined. Two-tailed Student’s t-tests were performed to determine the statistical significance for two-group comparisons (p < 0.05). The results shown are the averages and standard deviations of three independent assaysBack to article page