Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

Strain or plasmid

Characteristics

References

Strains

  

Pseudomonas putida

  

 DOT-T1E

RifR, TolR

[27]

 CM12-5

RifR, TolR, overproduces L-Phe

[25, 26]

Escherichia coli

  

 DH5α

Cloning host for pSEVA plasmids

[28]

Plasmids

  

 pSEVA238

Expression vector; oripBBR1, xylS-Pm, KmR

[29]

 pPALN

pSEVA238 derivative carrying pal genes from Nostoc punctiforme and Streptomyces maritimus

[26]

 pPALN_C1

pSEVA238 derivative carrying pal genes from Nostoc punctiforme and Streptomyces maritimus and psc1 gene

This work

 pSEVA632

Expression vector; oripBBR1, GmR

[29]

 pPSC1

pSEVA632 derivative carrying psc1 gene

This work

 pPSD1

pSEVA632 derivative carrying psd1 gene

This work

  1. RifR, rifampicin-resistant; GmR, gentamycin-resistant; KmR, kanamycin-resistant; TolR, toluene tolerant; L-Phe, L-phenylalanine; oripBBR1, origin of replication pBBR1; xylS-Pm, XylS-Pm regulator/promoter system