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Table 2 Biosynthesis of L-phenylalanine and trans-cinnamic acid by P. putida CM12-5 and P. putida CM12-5 (pPALN)

From: Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

 

[L-Phe] T 0 h (mg L−1)

[L-Phe] T 24 h (mg L−1)

[tCA] T 24 h (mg L−1)

CM12-5

0

217.2 ± 41.8

0

 

173.2 ± 0.9

356.9 ± 5.5

0

CM12-5 (pPALN)

0

141.8 ± 33.8

91.0 ± 11.2

 

173.6 ± 0.1

263.9 ± 41.0

119.7 ± 19.3

  1. Assays were done in M9 minimal medium with glucose as the sole C-source with and without the addition of 1 mM L-Phe (173 ± 1 mg/L)
  2. The concentrationof L-phenylalanine and trans-cinnamic acid was determined after 24 h’ cultivation
  3. The data in the table are the average and standard deviation of three independent assays
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Microbial Cell Factories

ISSN: 1475-2859

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