Fig. 3
From: Development of hybrid biomicroparticles: cellulose exposing functionalized fusion proteins
Obtaining the RGD- and AGF-derived concatemeric proteins in fusion with CBD. Panel (A) SDS-PAGE analysis of the purified CBD_RGD_X_His protein variants in 12% polyacrylamide gel. M, PageRuler Plus Prestained Protein Ladder; lane 1, CBD_His; lane 2, CBD_RGD_10_His; lane 3, CBD-RGD_20_His; lane 4, CBD-RGD_30_His; lane 5, CBD_RGD_40_His. Panel (B) Western blot analysis of proteins described in panel A with the use of anti-polyHistidine antibodies. Panel (C) SDS-PAGE analysis of the obtained CBD_AGF_X_His protein variants in 12% polyacrylamide gel. M, PageRuler Plus Prestained Protein Ladder; lane 1, CBD; lane 2, CBD-AGF_10_His; lane 3, CBD-AGF_20_His; lane 4, CBD-AGF_30_His; lane 5, CBD-AGF_40_His. Panel (D) Western blot analysis of the proteins described in panel C with the use of anti-polyHistidine antibodies