Fig. 4

TEM image of the MCC complexes. Panel (A) MCC at a magnification 13 000 ×, scale 1 μm. Panel (B) MCC at a magnification 68 000 ×, scale 200 nm. Panel (C) MCC and CBD_AGF_30_His protein incubated 18 h at 4 °C, then washed 3 times with 1 × PBS and observed with TEM at 68 000 × magnification. Panel (D) MCC and CBD_RGD_20_His protein incubated 18 h at 4 °C, then washed 3 times with 1 × PBS and observed with TEM at 68 000 × magnification. Samples loaded onto a 300 mesh copper grid (Sigma), covered with 2% collodion, sprayed with carbon and stained with 2% uranyl acetate and visualised with a Tecnai G2 Spirit BioTWIN TEM set at 120 kV. Pictures were captured with a Veleta CCD camera