Fig. 2

Identification of proteins in fractions responsible for the observed cytostatic effect. (a) Diagram depicting the experimental set-up of identifying potentially active proteins in the F > 50 kDa fraction. (b) SDS-PAGE analysis of active fractions separated via different chromatography techniques. Bands excised for MS analysis are marked with red rectangles, with their corresponding molecular weight provided. (c) Proteins identified by MS within the excised SDS-PAGE bands, along with their respective emPAI index. (d) Gene Ontology biological process enrichment analysis performed with the ShinyGO package [59]. Visualized are the top 20 significantly enriched pathways. Pathways associated with glucose degradation and its metabolic processes are marked in red rectangles. Details of the analysis are summarized in the Materials and Methods section, and full results of GO enrichment analysis are presented in Supplementary Table 2. Analysis parameters: FDR cut-off: 0.05, pathway size: Min. 10, Max. 2000; removed redundancy, and abbreviated pathways. Fold Enrichment was defined as the percentage of genes above the FDR cut-off, belonging to a pathway, divided by the corresponding percentage in the background (MS hits in F > 50 kDa).