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Fig. 6 | Microbial Cell Factories

Fig. 6

From: The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase

Fig. 6

The enzymatic and cytostatic activity of the F > 50 kDa fraction is a unique feature of the Lc4 strain. (a) SDS-PAGE gel stained with PageBlue (Thermo Fisher) with protein profiles of F > 50 kDa fractions from Lc4, Lc2, and Lc3 L. lactis strains. (b) Growth rate constant of HT-29 (left) and HCT116 (right) cells treated with F > 50 kDa fractions from Lc4, Lc2, Lc3, and GM17 > 50 kDa (as a control) (n = 3 independent biological replicates). Data presented as a mean with min to max range. (c) Enzymatic activity of F > 50 kDa fractions from Lc4, Lc2, and Lc3 strains. Data presented as mean +/- SD from n = 3. (d) Stacked bar chart presenting the percentage of injured, dead, and live bacterial cells (cultivation condition described in the Materials and Methods section). Data are presented as a mean from n = 3 biological replicates (*p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001 from one-way ANOVA with Dunnet’s correction)

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Microbial Cell Factories

ISSN: 1475-2859

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