Fig. 5

BioLector cultivations of L. lactis B1629. Cultivations were carried out in BOH3-RWP under non-aerated conditions. M17 complex media was used [10 g L⁻¹ glucose, 0.2 M MES (pH 6.5)]. Cultivations were carried out at 30 °C, 600 rpm shaking frequency, 3 mm shaking diameter without humidity control. MgSO₄ and CaCl₂ were supplemented at final concentrations of 0.25 g L⁻¹ and 0.5 g L⁻¹, respectively. For backscatter and pH graph, solid lines show the mean of 6 biological replicates with the colored areas around representing standard deviation. Antimicrobial activities were determined in unicates using the pHluorin2 assay with sensor strain L. innocua LMG2785/pNZ-pHin2^Lm. pH 1–6 indicates the pH value at which culture broth was incubated prior to cell separation. All supernatants were set to pH 6.2 prior to antimicrobial activity measurement