Fig. 6

BioLector cultivations of L. lactis B1629. Cultivations were carried out in BOH3-RWP under non-aerated conditions. M17 complex media was used [10 g L⁻¹ glucose, 0.5% (v/v) Tween 80]. Cultivations were carried out at 30 °C, 600 rpm shaking frequency, 3 mm shaking diameter without humidity control. Antimicrobial activities were determined using the pHluorin2 assay with sensor strain L. innocua LMG2785/pNZ-pHin2^Lm. For backscatter and pH graph, solid lines show the mean of three biological replicates with the colored areas around representing standard deviation. Please note, that for MES concentration of 0, 0.1 and 0.2 the same BU values of 4 and 8 were measured for the three biological replicates, so that no standard deviation is visible. Statistical analysis was carried out via ANOVA with LSD post-hoc-test with α = 0.05. Significant differences are marked with *