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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Automated workflow for characterization of bacteriocin production in natural producers Lactococcus lactis and Latilactobacillus sakei

Fig. 7

Antimicrobial activities determined in unicates via pHluorin2 assay using L. innocua LMG2785/pNZ-pHin2Lm following BioLector cultivation of L. sakei A1608. Cultivations were carried out in BOH3-RWP under non-aerated conditions. MRS complex media was used. Cultivations were carried out at 30 °C, 600 rpm shaking frequency, 3 mm shaking diameter without humidity control. “inc. at pH 1” indicates incubation of biomass at a pH value of 1 prior to cell separation. "+ Tween 80" indicates supplementation of 0.5% (v/v) of Tween 80. The supernatant was set to pH 6.2 prior to antimicrobial activity measurement

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Microbial Cell Factories

ISSN: 1475-2859

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