Fig. 8

BioLector cultivations of L. sakei A1608. Cultivations were carried out in BOH3-RWP under non-aerated conditions. MRS complex media was used. Cultivations were carried out at 30 °C, 600 rpm shaking frequency, 3 mm shaking diameter without humidity control. Tween 80 was supplemented at final concentrations of 0.5% (v/v). YE: yeast extract was supplemented at a final concentrations of 20 g L⁻¹. MES: MES buffer (pH 6.5) was supplemented at a final concentrations of 0.1 M. For backscatter and pH graph, solid lines show the mean of initially 12 biological replicates with the colored areas around representing standard deviation. The number of replicates decrease by two every 4 h of cultivation time due to sacrifice sampling. pH measurement experienced fallouts, missing values were extrapolated as mean of closest prior and posterior measurement value. Antimicrobial activities were determined in unicates using the pHluorin2 assay with sensor strain L. innocua LMG2785/pNZ-pHin2^Lm