Fig. 1
Schematic overview of tested constructs. (A): General structure of a bacterial 5’UTR in the context of the CASPONTM platform construct. The T7 promotor is depicted as yellow arrow while the ribosomal binding site is illustrated as green rectangle. (B): Tested 5’UTR variants, their sequences and the corresponding total free interaction energies between ribosome and mRNA. Expression enhancer elements are depicted as pink rectangles. (C): N-terminal variants of the T7AC tag and their minimal free folding energies of nucleotides − 4 to + 37. The original T7AC tag sequence is depicted as well as the new T7ACrare variant using a combination of rare and non-rare codons in its 5’end. Synonymous codons were chosen, which exhibit a low GC content as well as being classified as rare, with an occurrence lower than 10 per 1000 codons in the host cell genome, whereby the focus was on the latter parameter