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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli

Fig. 2

Results of the 5’UTR variant cultivations. (A): Bacterial cell growth kinetics of the different variants in the induction phase compared to the calculated theoretical growth curve. (B): Measured relative transcript amounts in relation to the 16 S rRNA at 0, 4 and 7 h after induction. (C): For each individual construct the recombinant protein titre at the end of fermentation, the product formed per relative transcript amount and hour, as well as the corresponding interaction free energy is depicted

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