Fig. 3From: Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coliGrowth behaviour and soluble product formation kinetics of T7AC and T7ACrare tagged proteins in identical carbon-limited fed-batch fermentations. A to C show each a different protein. (A): PTH (B): hFGF2 (C): TNFα (D): direct comparison of specific recombinant protein titres at the end of fermentations. Solid bars represent the T7AC tagged variants, while hatched bars depict their T7ACrare tagged counterparts. Results of statistical tests are notated with asterisks (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). Raw data as well as the results of the t-tests are summarized in the supplementary information Table S5. Data of all sampling points can be found in the supplementary information Table S4Back to article page