Table 2 PB experimental design for the factors influencing biosurfactants production by Natrialba sp. BG1 and N3
Trials | Variables | Response*E24% | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | X5 | Actual | Predicted | Residual | ||||
BG1 | N3 | BG1 | N3 | BG1 | N3 | ||||||
1 | 1 | − 1 | − 1 | 1 | − 1 | 20 | 20 | 20 | 20 | 7.1 | 0 |
2 | 1 | 1 | − 1 | − 1 | 1 | 60 | 60 | 55 | 55 | 5 | 5 |
3 | 1 | 1 | 1 | − 1 | − 1 | 40 | 40 | 45 | 45 | − 5 | − 5 |
4 | − 1 | 1 | 1 | 1 | -1 | 1 | 1 | 1 | 1 | 5 | 5 |
5 | 1 | − 1 | 1 | 1 | 1 | 40 | 40 | 40 | 40 | 0 | 0 |
6 | − 1 | 1 | − 1 | 1 | 1 | 1 | 1 | 6 | 6 | − 5 | − 5 |
7 | − 1 | − 1 | 1 | − 1 | 1 | 60 | 20 | 60 | 40 | 0 | 0 |
8 | − 1 | − 1 | − 1 | − 1 | − 1 | 40 | 40 | 40 | 20 | 0 | 0 |
Variables | Code | Coded and actual levels | |
|---|---|---|---|
− 1 | + 1 | ||
Oil (g%) | X1 | 0.3 | 0.6 |
NaCl (g%) | X2 | 20 | 30 |
Casamino acids (g%) | X3 | 0.3 | 0.6 |
pH | X4 | 10 | 12 |
Inoculum size (mL%) | X5 | 2 | 4 |