Microbial Cell Factories

Table 3 BB factorial experimental design for the biosurfactants production by Natrialba sp. BG1 and N3

From: Production, characterization and biomedical potential of biosurfactants produced by haloalkaliphilic archaea from Wadi El-Natrun, Egypt

Trials	Variables			Response*E24%
	X1	X2	X3	Actual		Predicted		Residual
	X1	X2	X3	BG1	N3	BG1	N3	BG1	N3
1	0	0	0	50	46	50	45.67	0	0.33
2	0	0	0	50	45	50	45.67	0	− 0.67
3	0	− 1	1	25	30	22	27.13	3	2.88
4	0	1	-1	25	32	28	34.88	− 3	− 2.88
5	− 1	− 1	0	50	43	45.38	38.75	4.63	4.25
6	0	0	0	50	46	50	45.67	0	0.33
7	1	0	− 1	13	10	5.38	2.88	7.63	7.13
8	0	1	1	13	11	6.75	5.38	6.25	5.63
9	0	− 1	− 1	13	10	19.25	15.63	− 6.25	− 5.63
10	− 1	0	1	13	10	20.63	17.13	− 7.62	− 7.13
11	− 1	0	− 1	50	46	48.38	44.63	1.63	1.38
12	1	0	1	13	11	14.63	12.38	− 1.62	− 1.38
13	1	1	0	13	10	17.63	14.25	− 4.62	− 4.25
14	1	− 1	0	1	1	2.38	2.5	− 1.37	− 1.5
15	− 1	1	0	25	26	23.63	24.5	1.38	1.5

Variables	Code	Coded level and actual level
Variables	Code	− 1	0	+ 1
Inoculum size (mL%)	X1	4	5	6
pH	X2	10	11	12
NaCl (g%)	X3	20	25	30

Triple tests were performed, and the average of three reading was considered

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