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Table 1 HPLC analysis of FOS yield and composition after 10 h reaction

From: Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger

Strain

Glucose (%)

Fructose (%)

Sucrose (%)

GF2 (%)

GF3 (%)

GF4 (%)

FOS (%)

20611

32.16 (± 0.13)a

1.30 (± 0.09)

14.47 (± 0.18)

21.32 (± 1.30)

24.66 (± 1.27)

5.09 (± 0.06)

52.07 (± 0.61)

20611 + 

GOX

27.97 (± 0.21)

1.23 (± 0.06)

11.50 (± 0.15)

25.84 (± 0.50)

26.93 (± 1.28)

6.54 (± 0.07)

59.31 (± 0.27)

GOF-3

27.72 (± 0.25)

1.16 (± 0.13)

10.49 (± 0.61)

27.22 (± 0.31)

26.88 (± 1.01)

6.53 (± 0.02)

60.63 (± 0.36)

  1. All reactions were carried out in a 40% (W/V) sucrose solution at 40 °C, with shaking at 120 rpm, the enzyme dosage was 6 U per g sucrose for the parental strain and equal amounts of the transformant cells
  2. aValues in parentheses are standard errors