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Fig. 3 | Microbial Cell Factories

Fig. 3

From: RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoring

Fig. 3

RNA aptamer-based in vitro RuBisCO activity assay. RNA aptamer-based RuBisCO activity assay for detecting ATP hydrolysis to ADP during 3-PG → 1,3-BPG was developed. The ADP-specific sensor was tested with the cyanobacterial cell lysate containing 100 µg of total protein with or without external ATP addition to the reaction mixture in HEPES buffer (pH 7.4). Comparative ATP hydrolysis exhibited significant increase in fluorescence in the reaction mixture containing ATP over the one without ATP supplementation. The assay was performed in biological triplicates

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Microbial Cell Factories

ISSN: 1475-2859

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