Fig. 5

Comparative analysis of biochemical and fluorescence based assays. Results from the NADH-based biochemical assays using bicarbonate and CO₂ as inorganic C substrates, and the RNA-aptamer based in vitro fluorescence assays, were normalized as percent relative activities. The highest activity obtained with the biochemical assay using 5% CO₂ was normalized to be 100% (the bars). The corresponding correlation coefficients were compared for validating the methods, showing them in the range 0.95–1.00 (the dots), indicating strong correlation corresponding to increase in the activity with time. All the assays were performed in biological triplicates and statistically significant differences were obtained using the Student’s t-test, p < 0.05. *statistically significant difference; ns, no significant difference