Fig. 7

LysSYL disrupted mixed-species biofilms associated with S. aureus. Disruption of dual-species biofilms formed by (A) S. aureus USA300 /A. baumannii ATCC 19606 and (C) S. aureus USA300/A. baumannii ATCC AB2 inoculated in a ratio of 1:1, respectively. The bacteria in the mixed-species biofilms were confirmed by Gram staining (up panels). Untreated bacterial biofilms were subjected to Gram-staining. The 24 h-old mixed biofilms were treated for 1 h with various concentrations of LysSYL as indicated, VAN (64 µg/mL) and MEM (128 µg/mL for ATCC 19606 and 8.192 mg/mL for AB2) served as controls (bottom panels). Disruption of single-species biofilms of (B) A. baumannii ATCC 19606 and (D) A. baumannii AB2. Bacterial cells in the biofilms were shown by Gram staining (up panels). The 24 h biofilms were treated for 1 h with various concentrations of LysSYL, MEM served as control (bottom panels). The biofilm mass in each well was determined by crystal violet assay. Data were expressed as mean ± SD. The analyses were measured by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, and ns showed no significance relative to 0 µg/mL of LysSYL treatment