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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing

Fig. 1

Enzymatic activities in relation to S. cerevisiae growth on glucuronoxylan. (A) Comparison of the capacity of different xylanases to degrade GX on cellulose thin films, as quantified by a decrease in film layer thickness after treatment with sodium phosphate buffer for 10 min until application of xylanase to the film performed in biological triplicates. (B) Growth performance of the xylose-fermenting S. cerevisiae XXX strain over time when supplemented with different combinations of xylanase, β-xylosidase, α-methyl-glucuronidase and acetyl xylan esterase performed in biological triplicates. (C) Overview of genes and recombinant enzymes (Xyn11, XylA and Agu115) involved in GX depolymerization and the xylose metabolic pathway of the engineered XXX strain. OD Equivalent = Optical density normalized from S. cerevisiae growth in Delft/glucose medium in a Growth-Profiler 960. GH = glycoside hydrolase. GX = glucuronoxylan.

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Microbial Cell Factories

ISSN: 1475-2859

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